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KMID : 0613820170270020187
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Journal of Life Science
2017 Volume.27 No. 2 p.187 ~ p.193
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Antiinflammatory Activity of Solvent-partitioned Fractions from Atriplex gmelinii C. A. Mey. in LPS-stimulated RAW264.7 Macrophages
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Jeong Hee-Jeong
Kim Ho-Jun
Ju Eun-Sin
Lee Seul-Gi
Kong Chang-Suk
Seo Young-Wan
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Abstract
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As a part of ongoing research to elucidate and characterize antiinflammatory nutraceuticals, the crude extracts from Atriplex gmelinii C. A. Mey. and their solvent-partitioned fractions were tested for their antiinflammatory potential in lipopolysaccharide (LPS)-stimulated RAW 264.7 mouse macrophages. The crude extracts of A. gmelinii C. A. Mey. were fractioned according to polarity with n-hexane, 85% aqueous methanol (85% aq. MeOH), n-butanol, and H2O. Their antiinflammatory activities were investigated in LPS-induced inflammation in mouse macrophages by measuring nitric oxide (NO) generation and mRNA expression of inflammation mediators, namely, inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), interleukin-1β (IL-1β), and IL-6. As a result, we confirmed that the crude extracts of A. gmelinii C. A. Mey. inhibited LPS-stimulated NO production and mRNA expression of iNOS and COX-2 as important inflammatory factors. The inhibition of NO production through the downregulation of important inflammatory factors such as iNOS, COX-2, IL-1β, and IL-6 was found by treatment with all solvent-partitioned fractions. Among all tested fractions, 85% aq. MeOH showed the strongest antiinflammatory response. Based on the current results, A. gmelinii C. A. Mey. was suggested to possess natural antiinflammatory components, indicating that it could be used as a valuable source of antiinflammatory substances.
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KEYWORD
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Anti-inflammation, Atriplex gmelinii C.A.Mey., lipopolysaccharide, macrophages, nitric oxide
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